Plasmid Collections

The Plasmid Collection Stacks allow the archiving of plasmids and their host strains, the search for various parameters and the export of strain information as a text file. The program is available for boxes of 10x10 and 8x8 strains which are the most common formats to store strain collections below -70°C.

Hints for Use

To enter new strains, click the corresponding position in the freezing box (if that is already occupied, the strain data are displayed).

On entering strain characteristics, plasmid-coded genes are selected from a self-produced list of genes by clicking, the genotype of the bacterial host strain should be entered into the commentary field. The names of strains without plasmid are automatically assembled into a pop-up menu for easy selection as the "Host strain". Parent plasmid and cloned fragment are specified by entering their position (number) in the collection, by choosing from a list of search result, or by directly typing in their names. For the first two options, links are automatically created to the respective positions in the collection to allow viewing the parents and returning by a simple click (visiting the host strain is also possible). Alternate names of the plasmid, its origin and any commentary can be entered into text fields.

If a restriction map is available as a PICT* or MacPaint file, it can be linked via an automatically created alias (which in most cases keeps the connection even when the original file is moved) and displayed on the card. The aliasses are collected in a folder; the stack must be notified of changes (other name and/or position) of this folder with the button "New Alias Folder". If you also use my "Plasmid-Maker" stack as a database for plasmids (restriction sites, gene locations), you can display the restiction data of the respective plasmid with a click on a button.
*With "Plasmid-Maker" and Adobe Illustrator, creating PICT plasmid maps is fast and easy.

Comments can be added to each allele in the list of genes (coded enzyme, origin, phenotype of a mutant...) and for each box (useful if several researchers pool their collections, or if a certain box is reserved for a specific project).
Strains can be copied within the collection (it is often faster to copy a similar strain and edit the differences than to enter all data one by one) and between collections.
Plasmids can be extracted from the collection using various criteria (name, commentary, host strain, plasmid coded genes, strain genotype). Excerpts can be assembled by searching strains and assembling them in a result list, which can be modified by deleting or pasting strains. The data can be printed or exported as a text only file (e.g., for a dissertation or publication).

Search results can be stored and edited, several searches can be accumulated, the search results can be exported to a text file.

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Last edited: June 3, 1996 by KaiFr